Journal: Oxidative Medicine and Cellular Longevity

Article Title: Low Molecular Weight Fucoidan Inhibits Pulmonary Fibrosis In Vivo and In Vitro via Antioxidant Activity

doi: 10.1155/2022/7038834

Figure Lengend Snippet: (a) Immunofluorescence detection of Nrf-2, HO-1, NQO1, and ROS; (b) expression of Nrf-2, HO-1, and NQO1 tested by Western blot; (c) statistical expressions of Nrf-2, HO-1, and NQO1 by Western blot ( ∗ p < 0.05 vs. the PF group; # p < 0.05 vs. the Sham group); (d) immunohistochemical detection of Nrf-2, HO-1, and NQO1; (e) statistical expressions of Nrf-2, HO-1, and NQO1 by an immunohistochemical assay ( ∗ p < 0.05 and ∗∗ p < 0.01 vs. the PF group; # p < 0.05 and ## p < 0.01 vs. the Sham group).

Article Snippet: Antibodies for immunofluorescence (Nrf-2, HO-1, and NQO1) were produced by ABclonal (Woburn, USA).

Techniques: Immunofluorescence, Expressing, Western Blot, Immunohistochemical staining

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Slingshot homolog-1-mediated Nrf2 sequestration tips the balance from neuroprotection to neurodegeneration in Alzheimer's disease.

doi: 10.1073/pnas.2217128120

Figure Lengend Snippet: Fig. 1. SSH1 inhibits Nrf2/ARE target gene expression independent of SSH1 phosphatase activity. (A1) Schematic of the Nrf2 reporter construct pREP-8xARE- GFP-SV40-BFP. (A2) Schematic of SSH1 and SSH1-CS proteins showing the catalytic domain (CAT) and binding sites for cofilin and p62. (B) Representative images of HT22 cells coexpressing the Nrf2 reporter (green and blue), with vector control, myc-Nrf2, and/or Flag-SSH1 (red). (C) Quantification of Nrf2 reporter [one- way ANOVA, F (2, 218) = 18.62, P < 0.0001, post hoc Dunnett, ****P < 0.0001, **P = 0.0076, n = 15 to 20 images/condition/experiment from three experiments]. (D) Representative images of HT22 cells coexpressing the Nrf2 reporter (green and blue) and vector or Flag-SSH1 (red), ± 200 μM H2O2 (2 h). (E) Quantification of Nrf2 reporter [one-way ANOVA, F (2, 519) = 124.7, P < 0.0001, post hoc Dunnett, ****P < 0.0001, n = 15 to 20 images/condition/experiment from four experiments]. (F) Representative images of HT22 cells coexpressing the Nrf2 reporter (green and blue), vector, Flag-SSH1, or Flag-SSH1CS (red), ± 250 μM H2O2 (3 h). (G) Quantification of Nrf2 reporter [one-way ANOVA, F (3, 308) = 61.03, P < 0.0001, post hoc Dunnett, ****P < 0.0001, ns = not significant, n = 8 to 12 images/ condition/experiment from four experiments]. (H) Representative images of HT22 cells cotransfected with the Nrf2 reporter (green and blue) and control siRNA or SSH1 siRNA, stained for SSH1 (red), ± 250 μM H2O2 (3 h). (I) Quantification of Nrf2 reporter [one-way ANOVA, F (3, 855) = 41.31, P < 0.0001, post hoc Dunnett, ****P < 0.0001, n = 15 to 20 images/condition/experiment from four experiments]. (J) Representative immunoblots from lysates of HEK293T cells expressing vector or Flag-SSH1 ± 15 µM NaAsO2 (18 h). (K) Quantification of HMOX1 and NQO1 proteins [one-way ANOVA; HMOX1: F (2, 21) = 310.2, P < 0.0001; NQO1: F (2, 21) = 34.82, P < 0.0001; post hoc Dunnett, ****P < 0.0001, ***P < 0.001, *P < 0.05. n = 8 samples/condition]. (L) Representative immunoblots from lysates of HEK293T cells coexpressing vector or myc-Nrf2 plus vector, Flag-SSH1, or Flag-SSH1-CS. (M) Quantification of HMOX1 protein [one-way ANOVA, F (2, 6) = 32.61, P = 0.0006, post hoc Dunnett, ***P = 0.006, **P = 0.0022, ns = not significant, n = 3 samples/condition]. (N) Representative immunoblots from lysates of HEK293T cells transfected with control or SSH1 siRNA ± 15 µM NaAsO2 (18 h). (O) Quantification of HMOX1 protein [one-way ANOVA, F (2, 15) = 185.4, P < 0.0001, post hoc Dunnett, ****P < 0.0001, n = 6 samples/condition]. (P) Quantification of NQO1 protein [one-way ANOVA, F (2, 14) = 34.49, P < 0.0001, post hoc Dunnett, ****P < 0.0001, ns = not significant, n = 6 samples/condition].

Article Snippet: The following antibodies were used to probe target proteins: rabbit polyclonal anti- SSH1(ECM Biosciences, SP1711); mouse monoclonal anti- Nrf2 (3G7)(Novus Biologicals, AF4000); rabbit monoclonal anti- Keap1 (D6B12) (Cell Signaling Technologies, 8,047); rabbit monoclonal anti- SQSTM1/p62 (D10E10) (Cell Signaling Technologies, 7,695); mouse monoclonal anti- FLAG (M2) (Sigma- Aldrich, F3165); rabbit monoclonal anti- Nrf2 (D1Z9C) (Cell Signaling Technologies, 12,721); mouse monoclonal anti- Keap1 (1B4) (Abcam, ab119403); mouse monoclonal antiSSH1 (1A5C8) (Santa Cruz Biotech, sc- 517226); Alexa Fluor 594 goat anti- rabbit IgG (Invitrogen, A11037); Alexa Fluor 594 goat anti- mouse IgG (Invitrogen, A11032); and Alexa Fluor 488 goat anti- rabbit IgG (Invitrogen, A11034).

Techniques: Targeted Gene Expression, Activity Assay, Construct, Binding Assay, Plasmid Preparation, Control, Staining, Western Blot, Expressing, Transfection

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Slingshot homolog-1-mediated Nrf2 sequestration tips the balance from neuroprotection to neurodegeneration in Alzheimer's disease.

doi: 10.1073/pnas.2217128120

Figure Lengend Snippet: Fig. 3. AD and FTLD-tau brains exhibit excessive levels of inhibitory SSH1–Nrf2 and Keap1–Nrf2 interactions. (A) Representative images of HT22 cells transfected with GFP (green) and vector or Flag-SSH1, treated ± 8 µM NaAsO2 (14 h) and subjected to PLA for SSH1–Nrf2 (red). (B) Quantification of SSH1–Nrf2 PLA puncta area/cell [Brown-Forsythe and Welch ANOVA, F (3, 202.7) = 30.48, F (3, 183.2) = 62.13, P < 0.0001, post hoc Dunnett, ****P < 0.0001, n = 10 to 15 images/ condition/experiment from four experiments]. (C) Representative images of human frontal gyrus sections from nondementia, AD, and FTLD-tau cases showing DAPI (blue) and PLA for SSH1–Nrf2 (red) and Keap1–Nrf2 (red). (D and E) Quantification of SSH1–Nrf2 PLA area in (D) control vs. AD (two-tailed t test, t = 6.329, df = 156, ****P < 0.0001, n = 8 to 10 images/case from 7 to 8 case/condition) and (E) control vs. FTLD-tau (two-tailed t test, t = 2.353, df = 157, *P = 0.0199, n = 8 to 10 images/case from eight cases/condition). (F and G) Quantification of Keap1–Nrf2 PLA area in (F) control vs. AD (two-tailed t test, t = 3.371, df = 154, ***P = 0.0009; n = 8 to 10 images/case from 7 to 8 cases/condition) and (G) control vs. FTLD-tau (two-tailed t test, t = 4.752, df = 152; ****P < 0.0001; n = 8 to 10 images/ case from eight cases/condition).

Article Snippet: The following antibodies were used to probe target proteins: rabbit polyclonal anti- SSH1(ECM Biosciences, SP1711); mouse monoclonal anti- Nrf2 (3G7)(Novus Biologicals, AF4000); rabbit monoclonal anti- Keap1 (D6B12) (Cell Signaling Technologies, 8,047); rabbit monoclonal anti- SQSTM1/p62 (D10E10) (Cell Signaling Technologies, 7,695); mouse monoclonal anti- FLAG (M2) (Sigma- Aldrich, F3165); rabbit monoclonal anti- Nrf2 (D1Z9C) (Cell Signaling Technologies, 12,721); mouse monoclonal anti- Keap1 (1B4) (Abcam, ab119403); mouse monoclonal antiSSH1 (1A5C8) (Santa Cruz Biotech, sc- 517226); Alexa Fluor 594 goat anti- rabbit IgG (Invitrogen, A11037); Alexa Fluor 594 goat anti- mouse IgG (Invitrogen, A11032); and Alexa Fluor 488 goat anti- rabbit IgG (Invitrogen, A11034).

Techniques: Transfection, Plasmid Preparation, Control, Two Tailed Test

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Slingshot homolog-1-mediated Nrf2 sequestration tips the balance from neuroprotection to neurodegeneration in Alzheimer's disease.

doi: 10.1073/pnas.2217128120

Figure Lengend Snippet: Fig. 4. Ssh1 elimination increases nuclear Nrf2, reduces oxidative injury, and alleviates AD pathology. (A) Representative images of the cortex from 7-mo-old WT, P301S, and P301S;Ssh1−/− mice stained for Nrf2 (green) and DAPI (blue). White boxes serially magnified to the right. (B) Quantification of nuclear/cytoplasmic Nrf2 intensity [one-way ANOVA, F (2, 134) = 13.06, P < 0.0001, post hoc Dunnett, ****P < 0.0001, **P = 0.0013, n = 10 to 12 images/mouse from four mice/genotype]. (C) Representative images of the cortex and hippocampus (CA3) stained for 8-OHdG (green) and DAPI (blue) from 7-mo-old WT, P301S, and P301S;Ssh1−/− mice. (D and E) Quantification of 8-OHdG intensity in the (D) cortex [one-way ANOVA, F (2, 134) = 37.79, P < 0.0001, post hoc Dunnett, ****P < 0.0001, n = 10 to 13 images/mouse from four mice/genotype) and (E) hippocampus [one-way ANOVA, F (2, 70) = 17.85, P < 0.0001, post hoc Dunnett, ****P < 0.0001, ***P = 0.0003, n = 5 to 7 images/mouse from four mice/genotype]. (F) Representative images of the cortex from 8-mo-old WT, APP/PS1, and APP/PS1;Ssh1−/− mice stained for 8-OHdG (red) and DAPI (blue). (G) Quantification of 8-OHdG intensity [one-way ANOVA, F (2, 181) = 36.83, P < 0.0001, post hoc Dunnett, ****P < 0.0001, n = 10 to 12 images/mouse from four mice/genotype]. (H) Representative images of silver staining of the cortex and hippocampus from 7-mo-old WT, P301S, and P301S;Ssh1−/− mice. Red arrows indicate silver-positive degenerating axons. (I and J) Quantification of silver-positive axons in the (I) cortex [one-way ANOVA, F (2, 21) = 14.5, P = 0.0001, post hoc Dunnett, ***P < 0.0005, n = 6 to 10 mice/genotype] and (J) hippocampus [one-way ANOVA, F (2, 21) = 7.545, P = 0.0034, post hoc Dunnett, **P < 0.0081, n = 6 to 10 mice/genotype]. (K) Representative images of the cortex and hippocampus (CA3) stained for pS199/202-tau (green) and DAPI (blue) from 7-mo-old P301S and P301S;Ssh1−/− mice. (L and M) Quantification of pS199/202-tau intensity in the (L) cortex (two-tailed t test, t = 13.85, df = 221, ****P < 0.0001, n = 20 to 30 images/mouse from four mice/genotype) and (M) hippocampus (two-tailed t–test, t = 8.147, df = 104, ****P < 0.0001, n = 13 to 18 images/mouse from four mice/genotype). (N) Representative images of the cortex and hippocampus stained for Aβ (green) and DAPI (blue) from 8-mo-old WT, APP/PS1, and APP/PS1;Ssh1−/− mice. (O) Quantification of Aβ intensity in the cortex (two-tailed t test, t = 2.784, df = 76, **P = 0.0068, n = 6 to 8 images/mouse from 4 to 6 mice/genotype).

Article Snippet: The following antibodies were used to probe target proteins: rabbit polyclonal anti- SSH1(ECM Biosciences, SP1711); mouse monoclonal anti- Nrf2 (3G7)(Novus Biologicals, AF4000); rabbit monoclonal anti- Keap1 (D6B12) (Cell Signaling Technologies, 8,047); rabbit monoclonal anti- SQSTM1/p62 (D10E10) (Cell Signaling Technologies, 7,695); mouse monoclonal anti- FLAG (M2) (Sigma- Aldrich, F3165); rabbit monoclonal anti- Nrf2 (D1Z9C) (Cell Signaling Technologies, 12,721); mouse monoclonal anti- Keap1 (1B4) (Abcam, ab119403); mouse monoclonal antiSSH1 (1A5C8) (Santa Cruz Biotech, sc- 517226); Alexa Fluor 594 goat anti- rabbit IgG (Invitrogen, A11037); Alexa Fluor 594 goat anti- mouse IgG (Invitrogen, A11032); and Alexa Fluor 488 goat anti- rabbit IgG (Invitrogen, A11034).

Techniques: Staining, Silver Staining, Two Tailed Test

Journal: PLoS ONE

Article Title: Electroacupuncture Ameliorates Acute Renal Injury in Lipopolysaccharide-Stimulated Rabbits via Induction of HO-1 through the PI3K/Akt/Nrf2 Pathways

doi: 10.1371/journal.pone.0141622

Figure Lengend Snippet: β-actin was monitored as the internal standard to ensure similar gel loading of the starting materials in each sample. Blot images were cropped for comparison (E). Electroacupuncture treatment significantly increased the levels of phospho-Akt protein, HO-1 protein, Nrf2 total and nucleoprotein compared with group L or group SEL. In wortmannin-treated rabbits, phosphorylation of Akt was significantly decreased while HO-1 protein, Nrf2 total and nucleoprotein was slightly decreased compared with group EL. All values were expressed as mean ±SD (n = 10; *P<0 . 05; **P<0 . 01 , using one-way ANOVA and the Bonferroni test for multiple comparisons). The blots were representative of three independent experiments.

Article Snippet: After protein blockade, the sections were incubated overnight at 4°Cwith the polyclonal Nrf2 primary antibody conjugated to FITC (dilution 1:150, Biorbyt, UK).

Techniques: Comparison, Phospho-proteomics

Journal: PLoS ONE

Article Title: Electroacupuncture Ameliorates Acute Renal Injury in Lipopolysaccharide-Stimulated Rabbits via Induction of HO-1 through the PI3K/Akt/Nrf2 Pathways

doi: 10.1371/journal.pone.0141622

Figure Lengend Snippet: (original magnification×400): (A) The pictures of immunofluorescence staining. Green stands for Nrf2-FITC stained sections, while blue stands for images of DAPI stained nuclei. The overlay color of blue staining in nucleus, accompanied with green staining both in cytoplasm and nucleus was considered to be positive. (B) Quantification of nuclear localization of Nrf2 protein among six groups. Electroacupuncture protocols dramatically increased translocation of Nrf2 from cytoplasm into nucleus compared with group L or SEL. To some degree, pretreatment with wortmannin counteracted nuclear accumulation of Nrf2 protein, while wortmannin alone had no effects. Data were representative of three independent experiments. Values were expressed as mean ± SD (n = 10; **P<0 . 01 , using one-way ANOVA and the Bonferroni test for multiple comparisons).

Article Snippet: After protein blockade, the sections were incubated overnight at 4°Cwith the polyclonal Nrf2 primary antibody conjugated to FITC (dilution 1:150, Biorbyt, UK).

Techniques: Immunofluorescence, Staining, Translocation Assay

Journal: Scientific Reports

Article Title: RNA sequencing of corneas from two keratoconus patient groups identifies potential biomarkers and decreased NRF2-antioxidant responses

doi: 10.1038/s41598-020-66735-x

Figure Lengend Snippet: Canonical pathways significantly altered in KC and KJ. Grey bars show total number of genes in the pathway (scale on top X axis), with blue and red bars showing the numbers of down-regulated and up-regulated genes (scale on bottom X axis). The p value next to each bar was calculated for that pathway by the IPA Core Analysis. “Acute Phase” is the Acute Phase Response Signaling pathway; “NRF2-mediated” is NRF2-mediated Oxidative Stress Response pathway; “ECM related” is the Hepatic Fibrosis / Hepatic Stellate Cell Activation pathway; “Rheumatoid Arthritis” is the Role of Macrophages, Fibroblasts and Endothelial Cells in Rheumatoid Arthritis pathway; and, “Osteoarthritis” includes genes associated with osteoarthritis.

Article Snippet: The following primary antibodies were used from Biorbyt Research Products: NRF2 (orb128433, 1:50), KEAP1 (orb48426, 1:100), Hsp40 (orb520080, 1:100), and GAS1 (orb414757, 1:100) and RXRA antibody were used from Novus Biologicals (NBP2–75653, 1:50).

Techniques: Activation Assay

Journal: Scientific Reports

Article Title: RNA sequencing of corneas from two keratoconus patient groups identifies potential biomarkers and decreased NRF2-antioxidant responses

doi: 10.1038/s41598-020-66735-x

Figure Lengend Snippet: NRF2 target genes significantly altered in KCN corneas. ( A ) Decreased NRF2 target gene expression in KC and KJ RNA seq. ( B ) Mechanism of NRF2 regulation by KEAP1; the latter binds to NRF2 and CUL3 for NRF2-ubiquitination and degradation to maintain low levels of NRF2 under homeostatic conditions. Under oxidative stress, KEAP1 dissociates from NRF2 allowing its increase and upregulation of target genes.

Article Snippet: The following primary antibodies were used from Biorbyt Research Products: NRF2 (orb128433, 1:50), KEAP1 (orb48426, 1:100), Hsp40 (orb520080, 1:100), and GAS1 (orb414757, 1:100) and RXRA antibody were used from Novus Biologicals (NBP2–75653, 1:50).

Techniques: Targeted Gene Expression, RNA Sequencing, Ubiquitin Proteomics

Journal: Scientific Reports

Article Title: RNA sequencing of corneas from two keratoconus patient groups identifies potential biomarkers and decreased NRF2-antioxidant responses

doi: 10.1038/s41598-020-66735-x

Figure Lengend Snippet: KEAP1 and NRF2 immunostaining in DN and KCN corneas. ( A ) KEAP1 staining is decreased in KCN corneas, with focally increased staining in some basal epithelial cells (inset), whereas in DN corneas KEAP1 shows staining of all epithelial layers (inset). ( B ) NRF2 shows very little to no staining of KCN corneas and these were all cytoplasmic (inset), while DN sections show stronger staining of epithelial cells and some nuclear staining (inset) DAPI nuclear staining shown in blue. IF staining of additional KCN and DN cornea sections are shown in Supplemental Fig. . Scale bar: 50 µm.

Article Snippet: The following primary antibodies were used from Biorbyt Research Products: NRF2 (orb128433, 1:50), KEAP1 (orb48426, 1:100), Hsp40 (orb520080, 1:100), and GAS1 (orb414757, 1:100) and RXRA antibody were used from Novus Biologicals (NBP2–75653, 1:50).

Techniques: Immunostaining, Staining

Journal: Bioengineered

Article Title: Long noncoding RNA BBOX1-AS1 promotes the progression of gastric cancer by regulating the miR-361-3p/Mucin 13 signaling axis.

doi: 10.1080/21655979.2022.2072629

Figure Lengend Snippet: Figure 5. MUC13 was targeted by miR-361-3p. (a) MUC13 and CLDN4 were predicted and screened as the target genes of miR-361- 3p using GEPIA and ENCORI. (b) RNA pull-down analysis on the interaction between MUC13 and CLDN4 of miR-361-3p. **P < 0.001 vs Bio-NC. (c) The binding sites of MUC13 on miR-361-3p were predicted by StarBase. (d) The luciferase reporter analysis on the relationship between miR-361-3p and MUC13. **P < 0.001 vs miR-NC. (e, f) The expression level of MUC13 in GC clinical samples (e) and GC cell lines (f) was detected by qRT-PCR. (g) Pearson analysis revealed the expression relationship between miR-361-3p and MUC13.

Article Snippet: These membranes were incubated with primary antibodies against GAPDH (1:1,000; #bs-10900 R; Bioss, Beijing, China) and MUC13 (1:1,000; #bs-1,074 R; Bioss, Beijing, China) at 4°C overnight and then incubated with the secondary antibody of horseradish peroxidase-conjugated goat anti-rabbit IgG (1:5,000; #ab6721; Abcam) for 1 h at 25°C.

Techniques: Binding Assay, Luciferase, Expressing, Quantitative RT-PCR

Journal: Bioengineered

Article Title: Long noncoding RNA BBOX1-AS1 promotes the progression of gastric cancer by regulating the miR-361-3p/Mucin 13 signaling axis.

doi: 10.1080/21655979.2022.2072629

Figure Lengend Snippet: Figure 6. MiR-361-3p knockdown promoted GC progression by targeting MUC13. (a) The expression level of miR-361-3p was evaluated in groups of si-NC, inhibitor-NC, inhibitor, si-MUC13, and si-MUC13+ inhibitor by qRT-PCR. (b-d) The cell proliferation (b), invasion (c), and apoptosis (d) were measured in groups of si-NC, inhibitor-NC, inhibitor, si-MUC13, and si-MUC13+ inhibitor using CCK-8, Transwell and flow cytometry assays, respectively. **P < 0.001 vs si-NC, &&P < 0.001 vs inhibitor-NC. ##P < 0.001 vs si-lnc +inhibitor.

Article Snippet: These membranes were incubated with primary antibodies against GAPDH (1:1,000; #bs-10900 R; Bioss, Beijing, China) and MUC13 (1:1,000; #bs-1,074 R; Bioss, Beijing, China) at 4°C overnight and then incubated with the secondary antibody of horseradish peroxidase-conjugated goat anti-rabbit IgG (1:5,000; #ab6721; Abcam) for 1 h at 25°C.

Techniques: Knockdown, Expressing, Quantitative RT-PCR, CCK-8 Assay, Flow Cytometry

Journal: Antioxidants & Redox Signaling

Article Title: Pterostilbene Decreases the Antioxidant Defenses of Aggressive Cancer Cells In Vivo : A Physiological Glucocorticoids- and Nrf2-Dependent Mechanism

doi: 10.1089/ars.2015.6437

Figure Lengend Snippet: Effect of Pter treatment on nuclear Nrf2 and its transcription activity in melanoma-bearing mice. (A) Expression of different Nrf2-dependent genes in A2058-RFP, MeWo-RFP, and MelJuso-RFP cells, isolated by laser microdissection (as indicated under the section) from in vivo growing tumors 35 days after tumor inoculation, was compared versus 48 h cultured HEMa-LP cells (*Significantly different p < 0.05). (B) Expression of the same genes was compared in in vivo growing A2058-RFP, MeWo-RFP, and MelJuso-RFP cells treated and untreated with Pter (as in ) (*Significantly different p < 0.05). All data for (A) and (B) , expressing fold change (quantitative RT-PCR, see under the section for calculations), show mean values ± SD for five to six different experiments. No significant differences in expression of the genes displayed were found when control A2058, MeWo, and MelJuso cells and their RFP counterparts were compared under in vitro conditions (not shown). (C) Nuclear accumulation of Nrf2 from in vivo growing A2058-RFP, MeWo-RFP, and MelJuso-RFP cells was measured by Western blotting (no significant differences were found when these data were compared with those found in control A2058, MeWo, and MelJuso cells, not shown) (mean values ± SD for five different experiments, *Significantly different p < 0.01, comparing Pter treatment vs . controls). (D) Effect of Nrf2 overexpression (see under the section) on the tumor growth of control and Pter-treated (as in ) A2058-bearing mice. Results obtained in these cells transfected with lentiviral vector not harboring any gene (negative control) were not different from control values (not shown). Data are mean values ± SD from six to seven different experiments (*Significantly different p < 0.01, comparing Pter treatment vs . controls; no significant differences were found when A2058/Tet-Nrf2 cells, ± Pter treatment, were compared with control A2058 untreated cells). Nrf2, nuclear factor (erythroid-derived 2)-like 2. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars

Article Snippet: Mouse monoclonal primary antibodies against Nrf2 were purchased from Abcam.

Techniques: Activity Assay, Expressing, Isolation, Laser Capture Microdissection, In Vivo, Cell Culture, Quantitative RT-PCR, Control, In Vitro, Western Blot, Over Expression, Transfection, Plasmid Preparation, Negative Control, Derivative Assay

Journal: Antioxidants & Redox Signaling

Article Title: Pterostilbene Decreases the Antioxidant Defenses of Aggressive Cancer Cells In Vivo : A Physiological Glucocorticoids- and Nrf2-Dependent Mechanism

doi: 10.1089/ars.2015.6437

Figure Lengend Snippet: Effect of Pter Treatment on Different Nrf2- and Redox State-Related Enzyme Activities and Metabolites in Melanoma Cells Growing In Vivo

Article Snippet: Mouse monoclonal primary antibodies against Nrf2 were purchased from Abcam.

Techniques: